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CD8b Monoclonal antibody, PBS Only

CD8b Monoclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B3B8

Cat no : 68432-1-PBS

Synonyms

CD8B, CD8b molecule, CD8B1, Leu2, Ly3, LYT3

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD8b Monoclonal antibody (68432-1-Ig)

    IHC staining of human breast cancer using 68432-1-Ig (same clone as 68432-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68432-1-Ig (CD8b antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68432-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CD8b Monoclonal antibody (68432-1-Ig)

    IHC staining of human breast cancer using 68432-1-Ig (same clone as 68432-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68432-1-Ig (CD8b antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68432-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68432-1-PBS targets CD8b in IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD8b fusion protein Ag16925
Full Name CD8b molecule
Calculated Molecular Weight 243 aa, 27 kDa
GenBank Accession NumberBC100913
Gene Symbol CD8B
Gene ID (NCBI) 926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 22-171 aa of human CD8b, the beta chain of CD8 molecule.