CoraLite® Plus 647 Anti-Mouse CD8a (5H10-1)

CD8a Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, lambda

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

5H10-1

Cat no : CL647-65205

Synonyms

BB154331, CD8 antigen, alpha chain, Cd8a, Ly 2, Ly 35, Ly B, Lyt 2

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL647-65205

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.125 ug CoraLite® Plus 647 Anti-Mouse CD8a (CL647-65205, Clone: 5H10-1) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65205 targets CD8a in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen: Concanavalin A-stimulated BALB/c splenic T cells
• Full Name: CD8 antigen, alpha chain
• GenBank Accession Number: BC030679
• Gene Symbol: Cd8a
• Gene ID (NCBI): 12525
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).