Anti-Human CD8a (OKT8) Rabbit IgG Recombinant Antibody

CD8a Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT8

Cat no : 65506-1-RR

Synonyms

CD8, p32, OKT8, MAL, Leu2

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Validation Data Gallery

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  FC experiment of human PBMCs using 65506-1-RR

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8a Rabbit Recombinant Antibody (65506-1-RR, Clone:OKT8) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.1 ug Anti-Human CD8a Mouse Monoclonal Antibody (Clone:OKT8) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65506-1-RR

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8a Rabbit Recombinant Antibody (65506-1-RR, Clone:OKT8) or Rabbit IgG control PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65506-1-RR

  1X10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD8a Rabbit Recombinant Antibody (65506-1-RR, Clone:OKT8) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 or 0.1 ug Anti-Human CD8a Mouse Monoclonal Antibody (Clone:OKT8) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  Affinity and Kinetic Characterization of 65506-1-RR

  Biolayer interferometry (BLl) kinetic assays of 65506-1-RR against Human CD8a were performed. The affinity constant is 1.51 nM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65506-1-RR targets CD8a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human thymocytes
• Full Name: CD8a molecule
• Calculated Molecular Weight: 235 aa, 26 kDa
• GenBank Accession Number: BC025715
• Gene Symbol: CD8A
• Gene ID (NCBI): 925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).