CD8a Polyclonal antibody

CD8a Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 29896-1-AP

Synonyms

CD8, Ly B, Ly 35, Ly 2, CD8α

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Validation Data Gallery

• 

  WB analysis using 29896-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29896-1-AP (CD8a antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 7 publications below
• IF: See 1 publications below

Product Information

29896-1-AP targets CD8a in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD8a fusion protein Ag31942
• Full Name: CD8 antigen, alpha chain
• Calculated Molecular Weight: 27KD
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: NM_001081110
• Gene Symbol: Cd8a
• Gene ID (NCBI): 12525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 is composed of two disulfide-linked chains and can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Publications

Species	Application	Title
human	IHC	Front Immunol; Machine learning-based identification of tumor-infiltrating immune cell-associated model with appealing implications in improving prognosis and immunotherapy response in bladder cancer patients; Authors - Hualin Chen
mouse	IHC	Molecules; Immune Enhancement of Clam Peptides on Immunosuppressed Mice Induced by Hydrocortisone; Authors - Meibin Lv
mouse	IHC	J Exp Clin Cancer Res; Regnase-1 downregulation promotes pancreatic cancer through myeloid-derived suppressor cell-mediated evasion of anticancer immunity; Authors - Junya Okabe
mouse	IHC	Bioorg Chem; Discovery of selective and potent USP22 inhibitors via structure-based virtual screening and bioassays exerting anti-tumor activity; Authors - Yue Zhang
mouse	IHC	Biomed Pharmacother; FGF19/FGFR4 signaling contributes to hepatocellular carcinoma survival and immune escape by regulating IGF2BP1-mediated expression of PD-L1; Authors - Chaoqin Guo
mouse	IF	J Transl Med; Potential crosstalk between SPP1 + TAMs and CD8 + exhausted T cells promotes an immunosuppressive environment in gastric metastatic cancer; Authors - Yan Du