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CD81 Polyclonal antibody

CD81 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27855-1-AP

Synonyms

CD81, CD81 antigen, CD81 molecule, Cell and organelle markers, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28



Tested Applications

Positive WB detected inRaji cells, mouse brain tissue, rat brain tissue, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27855-1-AP targets CD81 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD81 fusion protein Ag27298
Full Name CD81 molecule
Calculated Molecular Weight 26 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC002978
Gene Symbol CD81
Gene ID (NCBI) 975
RRIDAB_2880995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD81 (also known as TAPA1or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as an essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Protocols

Product Specific Protocols
WB protocol for CD81 antibody 27855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury

Authors - Xun Wu
humanWB

EMBO J

Extracellular vesicles from Zika virus-infected cells display viral E protein that binds ZIKV-neutralizing antibodies to prevent infection enhancement

Authors - Fanfan Zhao
ratWB

J Nanobiotechnology

Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy

Authors - Junlai Wan
mouseWB

J Control Release

Engineered exosome as targeted lncRNA MEG3 delivery vehicles for osteosarcoma therapy.

Authors - Xin Huang
humanWB

Biomed Pharmacother

A 3D culture system improves the yield of MSCs-derived extracellular vesicles and enhances their therapeutic efficacy for heart repair

Authors - Ling Sun
humanWB

J Pathol

Inflammatory capacity of exosomes released in the early stages of acute pancreatitis predicts the severity of the disease.

Authors - Montserrat Carrascal