CD80/B7-1 Recombinant antibody

CD80/B7-1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241371E10

Cat no : 84137-5-RR

Synonyms

Cd80, CD80 antigen, Cd28l, B7.1, B7 1

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Validation Data Gallery

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  WB analysis of mouse spleen using 84137-5-RR

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84137-5-RR (CD80/B7-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84137-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84137-5-RR against Mouse CD80/B7-1 were performed. The affinity constant is 0.204 nM.

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Tested Applications

• Positive WB detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84137-5-RR is CD80/B7-1 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD80 antigen
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_009855.2
• Gene Symbol: Cd80
• Gene ID (NCBI): 12519
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)