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Mouse CD80/B7-1 Recombinant antibody, PBS Only

CD80/B7-1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241371E10

Cat no : 84137-5-PBS

Synonyms

Cd80, CD80 antigen, Cd28l, B7.1, B7 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using CD80/B7-1 Recombinant antibody (84137-5-RR)

    WB analysis of mouse spleen using 84137-5-RR (same clone as 84137-5-PBS)

    mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84137-5-RR (CD80/B7-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84137-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84137-5-RR

    Affinity and Kinetic Characterization of 84137-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84137-5-RR against Mouse CD80/B7-1 were performed. The affinity constant is 0.204 nM.

Product Information

The immunogen of 84137-5-PBS is CD80/B7-1 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD80 antigen
Calculated Molecular Weight35 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_009855.2
Gene Symbol Cd80
Gene ID (NCBI) 12519
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)