Anti-Rat CD80/B7-1 (3H5) Mouse IgG2a Recombinant Antibody, PBS Only

CD80/B7-1 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

Conjugate

Unconjugated

CloneNo.

3H5

Cat no : 65580-1-PBS

Synonyms

CD80, Cd80 molecule, B7-1, B7.1, B7 1

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Validation Data Gallery

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  FC experiment of rat splenocytes using 65580-1-MR (same clone as 65580-1-PBS)

  1x10^6 unstimulated or LPS-stimulated rat splenocytes were surface stained with 0.25 ug Anti-Rat CD80/B7-1 Mouse IgG2a Recombinant Antibody (65580-1-MR, Clone: 3H5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Rat CD45RA (OX-33). Cells were not fixed. This data was developed using the same antibody clone with 65580-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65580-1-MR (same clone as 65580-1-PBS)

  1x10^6 LPS-stimulated rat splenocytes were surface stained with 0.25 ug Anti-Rat CD80/B7-1 Mouse IgG2a Recombinant Antibody (65580-1-MR, Clone: 3H5) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 unstimulated rat splenocytes were surface stained with 0.25 ug Anti-Rat CD80/B7-1 Mouse IgG2a Recombinant Antibody (65580-1-MR, Clone: 3H5)(black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 65580-1-PBS in a different storage buffer formulation.

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Product Information

65580-1-PBS targets CD80/B7-1 in FC applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
• Full Name: Cd80 molecule
• Calculated Molecular Weight: 36 kDa
• GenBank Accession Number: XM_017597885
• Gene Symbol: Cd80
• Gene ID (NCBI): 25408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)