Anti-Human CD8 (SK1) Mouse IgG2a Recombinant Antibody, PBS Only

CD8 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

SK1

Cat no : 65630-1-PBS

Synonyms

CD8A, SK1, p32, MAL, Leu2

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using 65630-1-MR (same clone as 65630-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (SK1) Mouse IgG2a Recombinant Antibody (65630-1-MR, Clone: SK1) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65630-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65630-1-MR (same clone as 65630-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (SK1) Mouse IgG2a Recombinant Antibody (65630-1-MR, Clone: SK1) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65630-1-PBS in a different storage buffer formulation.

---

Product Information

65630-1-PBS targets CD8 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD8a molecule
• Calculated Molecular Weight: 235 aa, 26 kDa
• GenBank Accession Number: BC025715
• Gene Symbol: CD8A
• Gene ID (NCBI): 925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).