Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody, PBS Only

CD8 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHT4

Cat no : 65559-1-PBS

Synonyms

CD8A, T-cell surface glycoprotein CD8 alpha chain, p32, MAL, Leu2

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Validation Data Gallery

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  FC experiment of human PBMCs using 65559-1-MR (same clone as 65559-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (65559-1-MR, Clone: UCHT4) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65559-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65559-1-MR (same clone as 65559-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (65559-1-MR, Clone: UCHT4) (red) or Mouse IgG2a Isotype Control, and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65559-1-PBS in a different storage buffer formulation.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65559-1-PBS targets CD8 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Thymocytes and Sézary T cells
• Full Name: CD8a molecule
• Calculated Molecular Weight: 235 aa, 26 kDa
• GenBank Accession Number: BC025715
• Gene Symbol: CD8A
• Gene ID (NCBI): 925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).