Anti-Human CD71 (OKT9) Rabbit Recombinant Antibody, PBS only

CD71 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT9

Cat no : 65507-1-PBS

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

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Validation Data Gallery

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  FC experiment of human PBMCs using 65507-1-PBS

  1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65507-1-PBS

  1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65507-1-PBS

  1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (left) and CoraLite®647-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65507-1-PBS targets CD71 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human T-cell acute lymphoblastic leukemia cells
• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• GenBank Accession Number: BC001188
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C. Stable for one year after shipment.