1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65507-1-PBS
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65507-1-PBS
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Product Information
65507-1-PBS targets CD71 in FC applications and shows reactivity with human samples.
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.
FC experiment of human PBMCs using 65507-1-PBS
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65507-1-PBS
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)
1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD71 Recombinant antibody
Catalog Number
65507-1-PBS
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
