Anti-Mouse CD71 (RI7217)

• 

  FC experiment of C57BL/6 mouse bone marrow cells using 65289-1-Ig

  1X10^6 C57BL/6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse TER-119 (TER-119) and 0.5 ug Anti-Mouse CD71 (65289-1-Ig, Clone: RI7217) or 0.5 ug Rat IgG2a Isotype Control (2A3), and FITC anti-Rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

CD71 Monoclonal Antibody for FC

Cat No. 65289-1-Ig

Clone No.RI7217

Publications(2)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse and More (1)

Applications

IF, FC

Synonyms

Tfrc, RI7217, TFR, TFR1, TR

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Tested Applications

• Positive FC detected in: C57BL/6 mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 1 publications below

Product Information

65289-1-Ig targets CD71 in IF, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  DMSO induced Friend erythroleukemia 745.6

• Full Name: transferrin receptor
• GenBank Accession Number: BC054522
• Gene Symbol: CD71
• Gene ID (NCBI): 22042
• RRID: AB_3083747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q62351
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule, creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	FC	Cell Chem Biol; Anti-tumor immunotherapy using engineered bacterial outer membrane vesicles fused to lysosome-targeting chimeras mediated by transferrin receptor; Authors - Ling-Yan Su
human	IF	Cancer Discov; Functional reprogramming of neutrophils within the brain tumor microenvironment by hypoxia-driven histone lactylation; Authors - Alessio Ugolini