CD70 Monoclonal antibody

CD70 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A11A10

Cat no : 67749-1-Ig

Synonyms

CD27 L, CD27 ligand, CD27L, CD27LG, CD70, CD70 antigen, CD70 molecule, TNFSF7



Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

67749-1-Ig targets CD70 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD70 fusion protein Ag28070
Full Name CD70 molecule
Calculated molecular weight 21 kDa
Observed molecular weight30 kDa
GenBank accession numberBC000725
Gene symbol CD70
Gene ID (NCBI) 970
RRIDAB_2918518
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CD70 antibody 67749-1-IgDownload protocol
IHC protocol for CD70 antibody 67749-1-IgDownload protocol
IF protocol for CD70 antibody 67749-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Cancer

Special issue "The advance of solid tumor research in China": Multi-omics analysis based on 1311 clear cell renal cell carcinoma samples identifies a glycolysis signature associated with prognosis and treatment response

Authors - Xi Tian