B3GAT1 Monoclonal antibody, PBS Only

B3GAT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F4A11

Cat no : 66711-1-PBS

Synonyms

B3GAT1, GlcAT P, GLCATP, GlcUAT P, Glucuronosyltransferase P, HNK 1, HNK1, LEU7, NK 1

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 66711-1-Ig (same clone as 66711-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66711-1-Ig (same clone as 66711-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66711-1-Ig (B3GAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66711-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66711-1-PBS targets B3GAT1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: B3GAT1 fusion protein Ag13593
• Full Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 and 100 kDa
• GenBank Accession Number: BC010466
• Gene Symbol: B3GAT1
• Gene ID (NCBI): 27087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment. The calculated molecular weight of B3GAT1 is 38 kDa and 66711-1-Ig antibody detectes 100 kDa form in SDS-PAGE which might as a result of homodimer or glycosylation.