Anti-Mouse CD56 Rabbit Recombinant Antibody

CD56 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240936A4

Cat no : 98118-1-RR

Synonyms

Ncam1, N-CAM-1, NCAM 1, Ncam, E NCAM

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Validation Data Gallery

• 

  FC experiment of mouse bone marrow cells using 98118-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD56 Rabbit Recombinant Antibody (98118-1-RR, Clone: 240936A4) or 0.25 ug Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 98118-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD56 Rabbit Recombinant Antibody (98118-1-RR, Clone:240936A4) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98118-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98118-1-RR against Mouse CD56 were performed. The affinity constant is 9.50 nM.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98118-1-RR is CD56 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: neural cell adhesion molecule 1
• Calculated Molecular Weight: 119 kDa
• Gene Symbol: Ncam1
• Gene ID (NCBI): 17967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Neural cell adhesion molecule 1 (NCAM1, also known as CD56, NCAM, and MSK39) is a membrane-bound cell surface glycoprotein and a member of the immunoglobulin supergene family, which induces cell-to-cell interactions during embryonic development, cell migration, and organogenesis (PMID: 8013635; 1855476; 1391354). Mouse natural killer (NK) cells do not express the murine homolog of CD56 (PMID: 17100875). The molecular weight of NCAM1 changes from the 120 kDa isoform in normal tissue to the 140/180 kDa isoforms in tumors (PMID: 10086383).