CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody

CD5 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240244A7

Cat no : CL647-98038-2

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL647-98038-2

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3ε and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL647-98038-2, Clone:240244A7), or Isotype Control. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-98038-2

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL647-98038-2, Clone:240244A7) (red), or Isotype Control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98038-2 is CD5 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD5 antigen
• Calculated Molecular Weight: 54 kDa
• GenBank Accession Number: NM_007650.3
• Gene Symbol: Cd5
• Gene ID (NCBI): 12507
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).