Anti-Mouse CD5 Rabbit Recombinant Antibody, PBS Only

CD5 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240244A7

Cat no : 98038-2-PBS

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 98038-2-RR (same clone as 98038-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-2-RR, Clone: 240244A7) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone: 145-2C11). Cells were not fixed. This data was developed using the same antibody clone with 98038-2-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98038-2-RR (same clone as 98038-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-2-RR, Clone: 240244A7) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98038-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98038-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98038-2-RR against Mouse CD5 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 98038-2-PBS is CD5 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD5 antigen
• Calculated Molecular Weight: 54 kDa
• GenBank Accession Number: NM_007650.3
• Gene Symbol: Cd5
• Gene ID (NCBI): 12507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).