CD5 Recombinant antibody

CD5 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240244C2

Cat no : 83451-4-RR

Synonyms

Cd5, CD5 antigen, Ly 1, Ly 12, Ly A, Lyt 1

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Validation Data Gallery

• 

  WB analysis of EL-4 using 83451-4-RR

  EL-4 cells were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 83451-4-RR

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: EL-4 cells, mouse thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83451-4-RR is CD5 Fusion Protein expressed in E. coli.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD5 antigen
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 55 kDa, 67 kDa
• GenBank Accession Number: NM_007650.3
• Gene Symbol: Cd5
• Gene ID (NCBI): 12507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).