APC Anti-Human CD45 (F10-89-4)

CD45 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

FC

Conjugate

APC Fluorescent Dye

Publications

2

CloneNo.

F10-89-4

Cat no : APC-65064

Synonyms

L CA, GP180, F10-89-4, EC:3.1.3.48, CD45R

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Validation Data Gallery

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  FC experiment of human PBMCs using APC-65064

  1X10^6 human PBMCs were surface stained with 10 ul APC Anti-Human CD45 (APC-65064, Clone: F10-89-4) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using APC-65064

  1X10^6 human PBMCs were surface stained with 10 ul APC Anti-Human CD45 (APC-65064, Clone: F10-89-4) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

APC-65064 targets CD45 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified T cells from human lymph nodes
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• RRID: AB_2882966
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and a stabilizer.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

Species	Application	Title
	FC	Asian J Pharm Sci; Bone mesenchymal stem cell-derived exosomes involved co-delivery and synergism effect with icariin via mussel-inspired multifunctional hydrogel for cartilage protection; Authors - Jia Zeng
human	FC	Microsyst Nanoeng; From the teapot effect to tap-triggered self-wetting: a 3D self-driving sieve for whole blood filtration; Authors - Yuang Li