Anti-Human CD45 (F10-89-4)

CD45 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

F10-89-4

Cat no : 65064-1-Ig

Synonyms

L CA, GP180, F10-89-4, EC:3.1.3.48, CD45R

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65064-1-Ig

  1x10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD45 (F10-89-4) (65064-1-Ig, Clone: F10-89-4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65064-1-Ig targets CD45 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified T cells from human lymph nodes
• Full Name: protein tyrosine phosphatase, receptor type, C
• GenBank Accession Number: BC014239
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• RRID: AB_2918371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Borate buffered saline, pH 8.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).