FITC Plus Anti-Mouse CD44 (IM7)

CD44 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (2)

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

Publications

2

CloneNo.

IM7

Cat no : FITC-65117

Synonyms

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using FITC-65117

  1X10^6 mouse splenocytes were surface co-stained with Anti-Mouse CD3 and 0.5 ug FITC Anti-Mouse CD44 (FITC-65117, Clone:IM7) or 0.5 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

FITC-65117 targets CD44 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
• Full Name: CD44 antigen
• GenBank Accession Number: BC051388
• Gene Symbol: Cd44
• Gene ID (NCBI): 12505
• RRID: AB_2883787
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Publications

Species	Application	Title
mouse	FC	Small; The Effect of the Nanoparticle Shape on T Cell Activation.; Authors - Jiwon Oh
human,mouse	FC	Discov Oncol; Preclinical efficacy of CBR-5884 against epithelial ovarian cancer cells by targeting the serine synthesis pathway; Authors - Kunxiang Gong