CoraLite®555 Anti-Mouse CD44 (IM7)

CD44 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

IM7

Cat no : CL555-65117

Synonyms

AU023126, AW121933, AW146109, Cd44, CD44 antigen, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, HERMES, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, Ly 24, MC56, MDU2, MDU3, MIC4, MUTCH I, Pgp 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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Validation Data Gallery

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  FC experiment of mouse splenocytes using CL555-65117

  1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite®555 Anti-Mouse CD44 (CL555-65117, Clone: IM7) or 0.5 ug CoraLite® Plus 555 Rat IgG2b Isotype Control (LTF-2) (CL555-65211, Clone: LTF-2), and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65117 targets CD44 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Dexamethasone-induced myeloid leukemia M1 cells
• Full Name: CD44 antigen
• GenBank Accession Number: BC051388
• Gene Symbol: Cd44
• Gene ID (NCBI): 12505
• RRID: AB_2919666
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.