Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody, PBS Only

CD40L/CD154 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240743E6

Cat no : 98120-1-PBS

Synonyms

CD154, Cd40l, Tnfsf5, Cd40lg, CD40 ligand

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Validation Data Gallery

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  FC experiment of mouse CD3+ T cells using 98120-1-RR (same clone as 98120-1-PBS)

  1x10^6 untreated or PMA and ionomycin treated mouse CD3+ T cells were surface stained with 0.25 ug Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody (98120-1-RR, Clone: 240743E6) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed. This data was developed using the same antibody clone with 98120-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse CD3+ T cells using 98120-1-RR (same clone as 98120-1-PBS)

  1x10^6 PMA and ionomycin treated mouse CD3+ T cells were surface stained with 0.25 ug Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody (98120-1-RR, Clone: 240743E6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated mouse CD3+ T cells were surface stained with 0.25 ug Anti-Mouse CD40L/CD154 Rabbit Recombinant Antibody (98120-1-RR, Clone: 240743E6) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed. This data was developed using the same antibody clone with 98120-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98120-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98120-1-RR against Mouse CD40L/CD154 were performed. The affinity constant is 0.989 nM.

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Product Information

The immunogen of 98120-1-PBS is CD40L/CD154 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD40 ligand
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: NM_011616.2
• Gene Symbol: CD154
• Gene ID (NCBI): 21947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.