APC Anti-Human CD40 (G28.5)

CD40 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

G28.5

Cat no : APC-65103

Synonyms

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

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Validation Data Gallery

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  FC experiment of human peripheral blood lymphocytes using APC-65103

  1X10^6 human peripheral blood lymphocytes were surface stained with 0.20 ug APC-anti-human CD40 (APC-65103, clone G28.5) (red) or 0.20 ug APC-mouse IgG1 isotype control (blue). Cells were not fixed.

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  FC experiment of Raji using APC-65103

  1X10^6 Raji cells were surface stained with 5 ul APC Anti-Human CD40 (APC-65103, Clone: G28.5) (red) or Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes, Raji cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65103 targets CD40 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD40 molecule, TNF receptor superfamily member 5
• Calculated Molecular Weight: 277 aa, 31 kDa
• GenBank Accession Number: BC012419
• Gene Symbol: CD40
• Gene ID (NCBI): 958
• RRID: AB_2882993
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).