CD38 Recombinant antibody, PBS Only (Detector)

CD38 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241246B1

Cat no : 84070-2-PBS

Synonyms

ADPRC 1, ADP ribosyl cyclase 1, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase

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Validation Data Gallery

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  WB analysis using 84070-2-RR (same clone as 84070-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84070-2-RR (CD38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  WB analysis using 84070-2-RR (same clone as 84070-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84070-2-RR (CD38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00991-2

  Sandwich ELISA standard curve of MP00991-2, Human CD38 Recombinant Matched Antibody Pair - PBS only. 84070-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0110. 84070-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00991-2

  Serum of six individual healthy human donors was measured. The CD38 concentration of detected samples was determined to be 0.3 ng/mL with a range of ND-1.0 ng/mL.

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  Sample test of MP00991-2

  The mean CD38 concentration was determined to be 244.5 ng/mL in Daudi cell extract based on a 1.8 mg/mL extract load and 65.6 ng/mL in Raji cell extract based on a 1.2 mg/mL extract load.

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  IHC staining of human tonsillitis using 84070-2-RR (same clone as 84070-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-2-RR (CD38 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84070-2-RR (same clone as 84070-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-2-RR (CD38 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 84070-2-RR (same clone as 84070-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (84070-2-RR, Clone: 241246B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 84070-2-RR (same clone as 84070-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (84070-2-RR, Clone: 241246B1 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84070-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00991-1

  Cytometric bead array standard curve of MP00991-1, CD38 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84070-4-PBS. Detection antibody: 84070-2-PBS. Standard: Eg0110. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00991-2

  Cytometric bead array standard curve of MP00991-2, CD38 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84070-1-PBS. Detection antibody: 84070-2-PBS. Standard: Eg0110. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00991-3

  Cytometric bead array standard curve of MP00991-3, CD38 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84070-3-PBS. Detection antibody: 84070-2-PBS. Standard: Eg0110. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84070-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84070-2-RR against Human CD38 were performed. The affinity constant is 0.999 nM.

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Product Information

84070-2-PBS targets CD38 as part of a matched antibody pair:

MP00991-1: 84070-4-PBS capture and 84070-2-PBS detection (validated in Cytometric bead array)

MP00991-2: 84070-1-PBS capture and 84070-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00991-3: 84070-3-PBS capture and 84070-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD38 molecule
• Calculated Molecular Weight: 300 aa, 34 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC007964
• Gene Symbol: CD38
• Gene ID (NCBI): 952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).