Anti-Mouse CD34 Rabbit Recombinant Antibody

CD34 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240659B11

Cat no : 98069-1-RR

Synonyms

CD34 antigen, 240659B11

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Validation Data Gallery

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  FC experiment of C57BL/6 mouse bone marrow cells using 98069-1-RR

  1x10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD34 Rabbit Recombinant Antibody (98069-1-RR, Clone:240659B11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Isotype Control . Cells were not fixed.

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  Affinity and Kinetic Characterization of 98069-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98069-1-RR against Mouse CD34 were performed. The affinity constant is 3.45 nM.

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Tested Applications

• Positive FC detected in: C57BL/6 mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98069-1-RR is CD34 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD34 antigen
• Calculated Molecular Weight: 41 kDa
• GenBank Accession Number: NM_133654
• Gene Symbol: Cd34
• Gene ID (NCBI): 12490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.