CD33 Monoclonal antibody

CD33 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a









Cat no : 67135-2-Ig


CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3

Tested Applications

Positive WB detected inMo7e cells, THP-1 cells, U-937 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67135-2-Ig targets CD33 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD33 fusion protein Ag11402
Full Name CD33 molecule
Calculated Molecular Weight 364 aa, 40 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC028152
Gene Symbol CD33
Gene ID (NCBI) 945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.


Product Specific Protocols
WB protocol for CD33 antibody 67135-2-IgDownload protocol
Standard Protocols
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