CD33 Monoclonal antibody
CD33 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B3G4
Cat no : 67135-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Mo7e cells, THP-1 cells, U-937 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67135-2-Ig targets CD33 in WB, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD33 fusion protein Ag11402 |
Full Name | CD33 molecule |
Calculated Molecular Weight | 364 aa, 40 kDa |
Observed Molecular Weight | 67-70 kDa |
GenBank Accession Number | BC028152 |
Gene Symbol | CD33 |
Gene ID (NCBI) | 945 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
Protocols
Product Specific Protocols | |
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WB protocol for CD33 antibody 67135-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |