• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody, PBS Only

CD324 (E-cadherin) Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240159B4

Cat no : 98030-2-PBS

Synonyms

E-cadherin, CD324, CAM 120/80, Cadherin-1, Cadherin 1

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of MCF-7 cells using Anti-Human CD324 (E-cadherin) Rabbit Recombinant A (98030-2-RR)

    FC experiment of MCF-7 using 98030-2-RR (same clone as 98030-2-PBS)

    1x10^6 MCF-7 cells were surface stained with 0.25 ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-2-RR, Clone:240159B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells should be stained prior to fixation. This data was developed using the same antibody clone with 98030-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using Anti-Human CD324 (E-cadherin) Rabbit Recombinant A (98030-2-RR)

    FC experiment of HT-29 using 98030-2-RR (same clone as 98030-2-PBS)

    1x10^6 HT-29 cells were surface stained with 0.25 ug Anti-Human E-cadherin Rabbit Recombinant Antibody (98030-2-RR, Clone:240159B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells should be stained prior to fixation. This data was developed using the same antibody clone with 98030-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98030-2-RR

    Affinity and Kinetic Characterization of 98030-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98030-2-RR against Human E-cadherin were performed. The affinity constant is 0.204 nM.

Product Information

The immunogen of 98030-2-PBS is CD324 (E-cadherin) Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD324 (E-cadherin) fusion protein Ag14973
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated Molecular Weight 882 aa, 97 kDa
GenBank Accession NumberBC141838
Gene Symbol E-cadherin
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.