CD31 Recombinant antibody, PBS Only

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  WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

  Untreated and PNGase F-treated lysates of human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80530-1-RR (same clone as 80530-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80530-1-RR (CD31 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 80530-1-RR (same clone as 80530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80530-1-RR (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

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  IF Staining of bEnd.3 using 80530-1-RR (same clone as 80530-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (80530-1-RR, Clone: 4N13 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 80530-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80530-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80530-1-RR against Human CD31 were performed. The affinity constant is below 1 pM.

CD31 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 80530-1-PBS

Clone No.4N13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PECAM 1, PECA1, GPIIA', GPIIA, EndoCAM

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Product Information

80530-1-PBS targets CD31 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD31 fusion protein Ag1787
• Full Name: platelet/endothelial cell adhesion molecule
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC022512
• Gene Symbol: CD31
• Gene ID (NCBI): 5175
• ENSEMBL Gene ID: ENSG00000261371
• RRID: AB_2918900
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16284
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).