Anti-Human CD28 (CD28.2)

CD28 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

FC, IHC, Cell treatment

Conjugate

Unconjugated

Publications

3

CloneNo.

CD28.2

Cat no : 65099-1-Ig

Synonyms

CD28, CD28 molecule, Tp44

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using 65099-1-Ig

  1X10^6 human PBMCs were surface co-stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65099-1-Ig

  1X10^6 human PBMCs were surface stained with CL647 Plus Anti-Human CD3 and 0.2 ug Anti-Human CD28 (65099-1-Ig, Clone:CD28.2) with CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

65099-1-Ig targets CD28 in FC, IHC, Cell treatment applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD28 molecule
• Calculated Molecular Weight: 220 aa, 25 kDa
• GenBank Accession Number: BC093698
• Gene Symbol: CD28
• Gene ID (NCBI): 940
• RRID: AB_2918401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

Publications

Species	Application	Title
human	IHC	Cancer Cell Int; Comprehensive analysis of prognostic immune-related genes and drug sensitivity in cervical cancer.; Authors - Ya-Nan Pi
human		J Transl Med; Scoring model based on the signature of non-m6A-related neoantigen-coding lncRNAs assists in immune microenvironment analysis and TCR-neoantigen pair selection in gliomas; Authors - Wenbo Zhao
human	Cell treatment	Aging (Albany NY); An integrated bioinformatic investigation of focal adhesion-related genes in glioma followed by preliminary validation of COL1A2 in tumorigenesis; Authors - Guojun Yao