Anti-Mouse CD279 (PD-1) (RMP1-14)

CD279 (PD-1) Monoclonal Antibody for ELISA, Blocking

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

ELISA, Blocking

Conjugate

Unconjugated

CloneNo.

RMP1-14

Cat no : 65288-1-Ig

Synonyms

Ly101, PD 1, Pdc1, Pdcd1, programmed cell death 1

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Validation Data Gallery

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  Neutralization experiment of CD279 (PD-1) using 65288-1-Ig

  Mouse PD-L1 (Eg0985) was coated at 70 ng/well. Serial diluted HRP conjugated mouse PD-1 (Eg0918) was added for binding test (blue cureve, refer to bottom X - left Y). Blocking test was performed by mixing serial diluted Rat anti-mouse PD-1 antibody 65288-1-Ig with 1 ug/mL HRP conjugated mouse PD-1 (Eg0918) followed by same dose coated Mouse PD-L1 (Eg0985) . Typicall ND50 is around 1ug/mL.

• 

  Neutralization experiment of CD279 (PD-1) using 65288-1-Ig

  Mouse PD-1 (Eg0918) was coated at 70 ng/well. Serial diluted HRP conjugated mouse PD-L1 (Eg0985) was added for binding test (blue cureve, refer to bottom X - left Y). Blocking test was performed by mixing serial diluted Rat anti-mouse PD-1 antibody 65288-1-Ig with 1 ug/mL HRP conjugated mouse PD-L1 (Eg0985) followed by applyting to 70 ng/well coated Mouse PD-1 (Eg0918) . Typicall ND50 is around 1-5 ug/mL.

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  ELISA experiment of Mouse PD-1 protein using 65288-1-Ig

  ELISA test of 65288-1-Ig. Mouse PD-1 (Eg0918) was coated at 70 ng/well followed by blocking. Serial diluted Rat anti-Mouse PD-1 antibody 65288-1-Ig was appied and detected with HRP conjugated secondary antibody. Signal was developed by TMB substrate.

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Product Information

65288-1-Ig targets CD279 (PD-1) in ELISA, Blocking applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse PD-1 transfected BHK cells
• Full Name: programmed cell death 1
• GenBank Accession Number: BC119179
• Gene Symbol: PD-1
• Gene ID (NCBI): 18566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). RMP1-14 is a monoclonal antibody that targets the murine PD-1 protein and can be used in mouse models to block the reaction between PD-1 and PD-L1/PD-L2 (PMID: 14764726; 31832706).