PE Anti-Mouse ICOSLG/B7-H2/CD275 (HK5.3)

ICOSLG/B7-H2/CD275 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

HK5.3

Cat no : PE-65114

Synonyms

CD275, B7-H2, Icosl, icos ligand, ICOS L



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65114 targets ICOSLG/B7-H2/CD275 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse B7-RP1 transfected cell line
Full Name icos ligand
GenBank Accession NumberBC029227
Gene Symbol CD275
Gene ID (NCBI) 50723
RRIDAB_2883903
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD275, also known as B7-H2, ICOSLG or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. CD275 is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of CD275/ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

Protocols

Product Specific Protocols
FC protocol for PE ICOSLG/B7-H2/CD275 antibody PE-65114Download protocol
Standard Protocols
Click here to view our Standard Protocols