PE Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Antibody

CD26 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

BA5b

Cat no : PE-65574

Synonyms

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using PE-65574

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul PE Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Antibody (PE-65574, Clone: BA5b), or PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using PE-65574

  1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Antibody (PE-65574, Clone: BA5b) (red), or PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65574 targets CD26 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: A human T cell clone
• Full Name: dipeptidyl-peptidase 4
• Calculated Molecular Weight: 88 kDa
• GenBank Accession Number: BC013329
• Gene Symbol: DPP4
• Gene ID (NCBI): 1803
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).