Anti-Human Glycophorin A/CD235a (HIR2)

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  FC experiment of human peripheral blood using 65174-1-Ig

  100 ul human peripheral blood (diluted 1:15 with PBS) was surface stained with 0.2 ug Anti-Human CD235a (65174-1-Ig, Clone:HIR2) or Mouse IgG2b Isotype Control (65128-1-Ig, Clone: MPC-11) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed.

• 

  FC experiment of human peripheral blood using 65174-1-Ig

  100 ul human peripheral blood (diluted 1:15 with PBS) was surface stained with 0.2 ug Anti-Human CD235a (65174-1-Ig, Clone:HIR2) or Mouse IgG2b Isotype Control (65128-1-Ig, Clone: MPC-11) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed.

Glycophorin A/CD235a Monoclonal Antibody for FC

Cat No. 65174-1-Ig

Clone No.HIR2

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Synonyms

Glycophorin A, CD235a, Glycophorin-A, GPA, GPErik

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Tested Applications

• Positive FC detected in: human peripheral blood

Recommended dilution

• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65174-1-Ig targets Glycophorin A/CD235a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Synthetic peptide (Human, N-terminal)

• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: Glycophorin A
• Gene ID (NCBI): 2993
• RRID: AB_2918449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P02724
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)