CD200R1 Recombinant antibody

CD200R1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240979B6

Cat no : 83889-4-RR

Synonyms

OX2R, Mox2r, CD200R, CD200 receptor 1, 240979B6

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 83889-4-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83889-4-RR (CD200R1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83889-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83889-4-RR against Mouse CD200R1 were performed. The affinity constant is 4.26 nM.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83889-4-RR is CD200R1 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD200 receptor 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: NM_021325.3
• Gene Symbol: Cd200r1
• Gene ID (NCBI): 57781
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell surface glycoprotein CD200 receptor 1 (CD200R1) is also called CD200R, CRTR2, MOX2R, and OX2R, and belongs to the CD200R family. CD200R1 is an inhibitory receptor for the CD200/OX2 cell surface glycoprotein. CD200R1 is highly glycosylated, containing ten potential N-glycosylation sites (PMID: 15187158). CD200R1 limits inflammation by inhibiting the expression of pro-inflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. As an immune inhibitory molecule, CD200R1 binds to CD200, ultimately leading to the inhibition of classical activation of macrophages and microglia (PMID: 24588829).