CoraLite® Plus 488 Anti-Human CD200 Rabbit Recombinant Antibody

CD200 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230268E6

Cat no : CL488-98007

Synonyms

OX 2, MRC, MOX2, MOX1, CD200 molecule

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Validation Data Gallery

• 

  FC experiment of human PBMCs using CL488-98007

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD200 (CL488-98007, Clone: 230268E6) or Isotype Control, and 5 ul CoraLite® Plus 647 Anti-Human CD19 (HIB19) (CL647-65110, Clone: HIB19). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-98007 targets CD200 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD200 molecule
• Calculated Molecular Weight: 269 aa, 30 kDa
• GenBank Accession Number: BC022522
• Gene Symbol: CD200
• Gene ID (NCBI): 4345
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).