Anti-Human CD200 Rabbit Recombinant Antibody, PBS Only

CD200 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241347E1

Cat no : 98091-1-PBS

Synonyms

OX 2, MRC, MOX2, MOX1, CD200 molecule

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Validation Data Gallery

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  FC experiment of human PBMCs using 98091-1-RR (same clone as 98091-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98091-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98091-1-RR (same clone as 98091-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) (red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98091-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98091-1-RR (same clone as 98091-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD200 Rabbit Recombinant Antibody (98091-1-RR, Clone:241347E1) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. This data was developed using the same antibody clone with 98091-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98091-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98091-1-RR against Human CD200 were performed. The affinity constant is 1.15 nM.

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Product Information

98091-1-PBS targets CD200 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD200 molecule
• Calculated Molecular Weight: 269 aa, 30 kDa
• GenBank Accession Number: BC022522
• Gene Symbol: CD200
• Gene ID (NCBI): 4345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).