CD200 Polyclonal antibody

CD200 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14057-1-AP

Synonyms

OX 2 membrane glycoprotein, OX 2, MRC, MOX2, MOX1

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Validation Data Gallery

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  WB analysis of HEK-293 using 14057-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14057-1-AP (CD200 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 14057-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14057-1-AP (CD200 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14057-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14057-1-AP (CD200 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

14057-1-AP targets CD200 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD200 fusion protein Ag5045
• Full Name: CD200 molecule
• Calculated Molecular Weight: 269 aa, 30 kDa
• Observed Molecular Weight: 41-47 kDa
• GenBank Accession Number: BC022522
• Gene Symbol: CD200
• Gene ID (NCBI): 4345
• RRID: AB_2878004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

Publications

Species	Application	Title
	IF	Stem Cell Res Ther; Psoralen alleviates radiation-induced bone injury by rescuing skeletal stem cell stemness through AKT-mediated upregulation of GSK-3β and NRF2.; Authors - Bo-Feng Yin
	IHC	Pharmaceutics; Minoxidil Nanoparticles Targeting Hair Follicles Enhance Hair Growth in C57BL/6 Mice.; Authors - Yoshihiro Oaku
	IF	Bioact Mater; Microgel-based carriers enhance skeletal stem cell reprogramming towards immunomodulatory phenotype in osteoarthritic therapy; Authors - Pei-Lin Li