CD1e Polyclonal antibody

CD1e Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22463-1-AP

Synonyms

R2G1, R2, hCD1e, CD1e molecule

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Validation Data Gallery

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  WB analysis using 22463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1e antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 22463-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22463-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22463-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22463-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 22463-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 22463-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22463-1-AP (CD1E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22463-1-AP targets CD1e in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD1e fusion protein Ag18008
• Full Name: CD1e molecule
• Calculated Molecular Weight: 388 aa, 44 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC131693
• Gene Symbol: CD1E
• Gene ID (NCBI): 913
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD1e molecule (CD1e, also known as R2 and CD1A) is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin (PMID: 10948205). The discoverers of the CD1 locus originally separated CD1 proteins into group 1 (CD1a, CD1b, and CD1c) and group 2 (CD1d), based on amino acid sequence homology (PMID: 2467814). CD1e is an important modulator of group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability and the generation and persistence of CD1-lipid complexes (PMID: 21844346).