CD19 Monoclonal antibody

CD19 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

1C10A1

Cat no : 66298-1-Ig

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

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Validation Data Gallery

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  WB analysis of Raji using 66298-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66298-1-Ig

  Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  WB analysis of Ramos using 66298-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66298-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66298-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66298-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66298-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 66298-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (66298-1-Ig, Clone: 1C10A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, Ramos cells, Daudi cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

66298-1-Ig targets CD19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD19 fusion protein Ag19945
• Full Name: CD19 molecule
• Calculated Molecular Weight: 556 aa, 61 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC006338
• Gene Symbol: CD19
• Gene ID (NCBI): 930
• RRID: AB_2881681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Publications

Species	Application	Title
human	WB	Cancer Lett; Adenovirus-mediated specific tumor tagging facilitates CAR-T therapy against antigen-mismatched solid tumors.; Authors - Xinying Tang
human	IHC	Cancer Cell Int; Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.; Authors - Runzhi Huang
human	IHC	Int Immunopharmacol; The impact of tobacco exposure on tumor microenvironment and prognosis in lung adenocarcinoma by integrative analysis of multi-omics data; Authors - Xiaomin Lu
human	WB	In Vitro Cell Dev Biol Anim; The interaction of O-GlcNAc-modified NLRX1 and IKK-α modulates IL-1β expression in M1 macrophages.; Authors - Liqiong Chen
human	IF	J Cell Physiol; Upregulation of ENKD1 disrupts cellular homeostasis to promote lymphoma development; Authors - Ting Song
human	IHC,IF	Front Med (Lausanne); Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL; Authors - Xujuan Zhang