CD19 Monoclonal antibody

CD19 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


human and More (1)







Cat no : 66298-1-Ig


B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

Tested Applications

Positive WB detected inRaji cells, Ramos cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRaji cells
Positive FC detected inRaji cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:750-1:3000
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66298-1-Ig targets CD19 in WB,IHC,IF,FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD19 fusion protein Ag19945
Full Name CD19 molecule
Calculated Molecular Weight 556 aa, 61 kDa
Observed Molecular Weight 95 kDa
GenBank Accession NumberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.


Product Specific Protocols
WB protocol for CD19 antibody 66298-1-IgDownload protocol
IHC protocol for CD19 antibody 66298-1-IgDownload protocol
IF protocol for CD19 antibody 66298-1-IgDownload protocol
FC protocol for CD19 antibody 66298-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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