Anti-Mouse CD19 (1D3)

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  IF Staining of mouse spleen using 65290-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using CD19 antibody (65290-1-Ig, Clone: 1D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of C57BL/6 mouse splenocytes using 65290-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD19 (65290-1-Ig, Clone:1D3) or 0.5 ug Rat IgG2a Isotype Control (2A3), and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed.

CD19 Monoclonal Antibody for IF-Fro, FC

Cat No. 65290-1-Ig

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF-Fro, FC

Synonyms

CD19 antigen, 1D3

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Tested Applications

• Positive IF-Fro detected in: mouse spleen tissue
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65290-1-Ig targets CD19 in IF-Fro, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse CD19 Transfected Cell Line
• Full Name: CD19 antigen
• GenBank Accession Number: BC156767
• Gene Symbol: Cd19
• Gene ID (NCBI): 12478
• RRID: AB_3084916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).