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  • KD/KO Validated

CD18 Polyclonal antibody

CD18 Polyclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 10554-1-AP


CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7

Tested Applications

Positive WB detected inU-937 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inRAW 264.7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10554-1-AP targets CD18 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD18 fusion protein Ag0861
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 85-90 kDa
GenBank Accession NumberBC005861
Gene Symbol ITGB2
Gene ID (NCBI) 3689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).


Product Specific Protocols
WB protocol for CD18 antibody 10554-1-APDownload protocol
IHC protocol for CD18 antibody 10554-1-APDownload protocol
IF protocol for CD18 antibody 10554-1-APDownload protocol
FC protocol for CD18 antibody 10554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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