CoraLite® Plus 647 Anti-Human CD163 (GHI/61) Rabbit Recombinant Antibody

CD163 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

GHI/61

Cat no : CL647-65501

Synonyms

CD163, CD163 molecule

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65501

  1x10^6 human PBMCs were surface stained with FITC Anti-Human CD14 and 5 ul CoraLite® Plus 647 Anti-Human CD163 Rabbit Recombinant Antibody (CL647-65501, Clone:GHI/61) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65501

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD163 Rabbit Recombinant Antibody (CL647-65501, Clone:GHI/61) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL647-65501

  1x10^6 human PBMCs were surface stained with FITC Anti-Human CD14 and 5 ul CoraLite® Plus 647 Anti-Human CD163 Rabbit Recombinant Antibody (CL647-65501, Clone:GHI/61) or 5 ul CoraLite® Plus 647 Anti-Human CD163 Mouse Monoclonal Antibody (CL647-65169, Clone:GHI/61). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65501 targets CD163 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Glycoprotein preparation from hairy cell leukemia spleen
• Full Name: CD163 molecule
• Calculated Molecular Weight: 1156 aa, 125 kDa
• GenBank Accession Number: BC051281
• Gene Symbol: CD163
• Gene ID (NCBI): 9332
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).