Mouse CD163 Recombinant antibody, PBS Only (Capture/Detector)

CD163 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240233G5

Cat no : 83285-2-PBS

Synonyms

sCD163, M130, 240233G5

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00326-3

  Sandwich ELISA standard curve of MP00326-3, mouse CD163 Recombinant Matched Antibody Pair - PBS only. 83285-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Eg0794. 83285-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL..

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  Sample test of MP00326-3

  For the mouse lung supernatant cultured for 3 days, the mean mouse CD163 concentration was determined to be 154.4 pg/mL in ConA stimulated mouse lung supernatant.

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  Sample test of MP00326-3

  Serum of sixteen mice was measured. The CD163 concentration of detected samples was determined to be 92.2 ng/mL with a range of 46.2-146.5 ng/mL.

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  Cytometric bead array standard curve of MP00326-1

  Cytometric bead array standard curve of MP00326-1, MOUSE CD163 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83285-2-PBS. Detection antibody: 83285-1-PBS. Standard: Eg0794. Range: 0.313-40ng/mL.

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  Cytometric bead array standard curve of MP00326-2

  Cytometric bead array standard curve of MP00326-2, MOUSE CD163 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83285-2-PBS. Detection antibody: 83285-3-PBS. Standard: Eg0794. Range: 0.313-40ng/mL.

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Product Information

83285-2-PBS targets CD163 as part of a matched antibody pair:

MP00326-1: 83285-2-PBS capture and 83285-1-PBS detection (validated in Cytometric bead array)

MP00326-2: 83285-2-PBS capture and 83285-3-PBS detection (validated in Cytometric bead array)

MP00326-3: 83285-1-PBS capture and 83285-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD163 antigen
• Calculated Molecular Weight: 121 kDa
• GenBank Accession Number: NM_053094.2
• Gene Symbol: Cd163
• Gene ID (NCBI): 93671
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).