FITC Anti-Mouse CD16 / CD32 (2.4G2)

CD16 / CD32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

FITC Fluorescent Dye

Publications

4

CloneNo.

2.4G2

Cat no : FITC-65080

Synonyms

AI528646, CD16 / CD32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

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Validation Data Gallery

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  FC experiment of mouse splenocytes using FITC-65080

  1X10^6 C57BL/6 mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.25 ug FITC-Rat IgG2b isotype control (left) or 0.25 ug FITC Anti-Mouse CD16 / CD32 (FITC-65080, Clone: 2.4G2) (right). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 4 publications below

Product Information

FITC-65080 targets CD16 / CD32 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: Fc receptor, IgG, low affinity IIb
• GenBank Accession Number: BC038070
• Gene Symbol: Fcgr2b
• Gene ID (NCBI): 14130
• RRID: AB_2883765
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 498 nm / 526 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. The 2.4G2 antibody reacts with the extracellular domains of mouse CD16 (FcRIII) and CD32 (FcRII) (PMID: 90108; 8406898).

Publications

Species	Application	Title
mouse	FC	Int J Mol Sci; C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis; Authors - Xinyi Qu
mouse	FC	Pharm Biol; Schizandrin A ameliorates cognitive functions via modulating microglial polarisation in Alzheimer's disease mice.; Authors - Qi Wang
	FC	Neurotoxicology; Ursolic acid promotes microglial polarization toward the M2 phenotype via PPARγ regulation of MMP2 transcription; Authors - Yuye Wang
mouse	FC	Small; Relieving Macrophage Dysfunction by Inhibiting SREBP2 Activity: A Hypoxic Mesenchymal Stem Cells-Derived Exosomes Loaded Multifunctional Hydrogel for Accelerated Diabetic Wound Healing; Authors - Yan Shi