CD133 Monoclonal antibody

CD133 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2B8A2

Cat no : 66666-1-Ig

Synonyms

AC133, Antigen AC133, CD133, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,2,3,5,7, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4



Tested Applications

Positive WB detected inHT-29 cells, Caco-2 cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHT-29 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66666-1-Ig targets CD133 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD133 fusion protein Ag13327
Full Name prominin 1
Calculated molecular weight 97 kDa
Observed molecular weight 115 kDa, 80-90 kDa
GenBank accession numberBC012089
Gene symbol PROM1
Gene ID (NCBI) 8842
RRIDAB_2801586
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).

Protocols

Product Specific Protocols
WB protocol for CD133 antibody 66666-1-IgDownload protocol
IHC protocol for CD133 antibody 66666-1-IgDownload protocol
FC protocol for CD133 antibody 66666-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Exp Clin Cancer Res

ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma

Authors - Tong Xu
humanIHC

Mol Ther Oncolytics

ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer.

Authors - Hengqiang Zhao
humanWB,IHC,IF

Aging (Albany NY)

The critical role of peroxiredoxin-2 in colon cancer stem cells.

Authors - Linglong Peng
humanIF

Pharmacol Res

Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B.

Authors - Xiaomei Li
humanIHC

Int J Mol Sci

Promoter Methylation of Cancer Stem Cell Surface Markers as an Epigenetic Biomarker for Prognosis of Oral Squamous Cell Carcinoma

Authors - Yu Kyeong Han
mouseIF

Int J Mol Sci

Cell-Surface GRP78-Targeted Chimeric Antigen Receptor T Cells Eliminate Lung Cancer Tumor Xenografts

Authors - Shijie Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Udesh (Verified Customer) (08-16-2023)

Worked well in WB at 1:1000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Glioblastoma cells