Validation Data Gallery
|Positive WB detected in
|HT-29 cells, Caco-2 cells
|Positive IHC detected in
|human kidney tissue, human breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IHC : 1:500-1:2000
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66666-1-Ig targets CD133 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
|human, mouse, rat
|Host / Isotype
|Mouse / IgG1
|CD133 fusion protein Ag13327
|Calculated molecular weight
|Observed molecular weight
|115 kDa, 80-90 kDa
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. CD133 is a highly glycosylated protein with an apparent molecular weight of 115-120 kDa. After the treatment of the lysates with glycosidase, CD133 shifted to a protein with an apparent molecular weight of 80-90 kDa (PMID: 23150174; 20068153).
J Exp Clin Cancer Res
ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma
Mol Ther Oncolytics
ROS/KRAS/AMPK Signaling Contributes to Gemcitabine-Induced Stem-like Cell Properties in Pancreatic Cancer.
Aging (Albany NY)
The critical role of peroxiredoxin-2 in colon cancer stem cells.
Sinomenine hydrochloride suppresses the stemness of breast cancer stem cells by inhibiting Wnt signaling pathway through down-regulation of WNT10B.
Int J Mol Sci
Promoter Methylation of Cancer Stem Cell Surface Markers as an Epigenetic Biomarker for Prognosis of Oral Squamous Cell Carcinoma
Int J Mol Sci
Cell-Surface GRP78-Targeted Chimeric Antigen Receptor T Cells Eliminate Lung Cancer Tumor Xenografts
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (08-16-2023)
Worked well in WB at 1:1000