FITC Plus Anti-Mouse CD11b (M1/70)

CD11b Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse, Human and More (1)

Applications

IF, FC

Conjugate

FITC Plus Fluorescent Dye

Publications

11

CloneNo.

M1/70

Cat no : FITC-65055

Synonyms

CD11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

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Validation Data Gallery

• 

  FC experiment of mouse bone marrow cells using FITC-65055

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (M1/70) (FITC-65055, Clone:M1/70), and 0.25 ug APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (APC-65140, Clone: RB6-8C5), and 0.25 ug FITC Plus Rat IgG2b Isotype Control (LTF-2) (FITC-65211, Clone: LTF-2). Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-65055

  1X10^6 human PBMCs were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (FITC-65055, Clone: M1/70). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65055

  1X10^6 human PBMCs were surface stained with 0.25 ug FITC Plus Anti-Mouse CD11b (FITC-65055, Clone:M1/70). Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells, human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 3 publications below
• FC: See 7 publications below

Product Information

FITC-65055 targets CD11b in IF, FC applications and shows reactivity with Mouse, Human samples.

• Tested Reactivity: Mouse, Human
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes
• Full Name: integrin alpha M
• Calculated Molecular Weight: 127 kDa
• GenBank Accession Number: BC156991
• Gene Symbol: CD11b
• Gene ID (NCBI): 16409
• RRID: AB_2883745
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
		Cell Chem Biol; Small-molecule MHC-II inducers promote immune detection and anti-cancer immunity via editing cancer metabolism; Authors - Ling Huang
mouse	FC	Cell Commun Signal; Transfer of cGAMP from neuron to microglia activates microglial type I interferon responses after subarachnoid hemorrhage; Authors - Hanxiao Chang
		Neoplasia; Tumor microenvironment(TME) and single-source dual-energy CT(ssDECT) on assessment of inconformity between RECIST1.1 and pathological remission in neoadjuvant immunotherapy of NSCLC; Authors - Chao Sun
mouse	IF,FC	Int Immunopharmacol; Methionine enkephalin inhibited cervical carcinoma via apoptosis promotion and reduction of myeloid derived suppressor cell infiltrated in tumor.; Authors - Na Qu
mouse	FC	Cell Death Dis; Lactate secreted by PKM2 upregulation promotes Galectin-9-mediated immunosuppression via inhibiting NF-κB pathway in HNSCC.; Authors - Hanyue Chang
mouse	IF	Molecules; Tabersonine Inhibits the Lipopolysaccharide-Induced Neuroinflammatory Response in BV2 Microglia Cells via the NF-κB Signaling Pathway; Authors - Jiaotai Shi