CoraLite® Plus 647 Anti-Mouse CD11b (M1/70)

CD11b Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse, Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

M1/70

Cat no : CL647-65055

Synonyms

Cd11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

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Validation Data Gallery

• 

  FC experiment of mouse bone marrow cells using CL647-65055

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD11b (M1/70) (CL647-65055, Clone:M1/70), and 0.25 ug CoraLite® Plus 488 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (CL488-65140, Clone: RB6-8C5), and 0.25 ug CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2) (CL647-65211, Clone: LTF-2). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65055 targets CD11b in FC applications and shows reactivity with Mouse, Human samples.

• Tested Reactivity: Mouse, Human
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes
• Full Name: integrin alpha M
• GenBank Accession Number: BC156991
• Gene Symbol: CD11b
• Gene ID (NCBI): 16409
• RRID: AB_2883658
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).