Mouse CD107b / LAMP2 Recombinant antibody, PBS Only (Capture)

CD107b / LAMP2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241893G7

Cat no : 84474-3-PBS

Synonyms

CD107b, Lamp2, Mac3, Lamp 2, 241893G7

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Validation Data Gallery

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  WB analysis using 84474-3-RR (same clone as 84474-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84474-3-RR (CD107b / LAMP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84474-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84474-3-RR (same clone as 84474-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84474-3-RR (CD107b / LAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84474-3-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 84474-3-RR (same clone as 84474-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CD107b / LAMP2 antibody (84474-3-RR, Clone: 241893G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84474-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01356-2

  Cytometric bead array standard curve of MP01356-2, MOUSE LAMP-2/CD107b Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84474-3-PBS. Detection antibody: 84474-4-PBS. Standard: Eg2029. Range: 0.625-80 ng/mL.

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Product Information

84474-3-PBS targets CD107b / LAMP2 as part of a matched antibody pair:

MP01356-2: 84474-3-PBS capture and 84474-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: NM_001017959.2
• Gene Symbol: CD107b / LAMP2
• Gene ID (NCBI): 16784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.