CoraLite®555 Anti-Mouse CD107b / LAMP2 (ABL-93)

CD107b Monoclonal Antibody for IF

Host / Isotype

Rat / IgG2a

Reactivity

Mouse

Applications

IF

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

ABL-93

Cat no : CL555-65052

Synonyms

CD107b, Lamp 2, Lamp 2a, Lamp 2b, Lamp 2c, Lamp2, Mac3

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Validation Data Gallery

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  IF Staining of NIH/3T3 using CL555-65052

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 555 CD107b antibody (CL555-65052, Clone: ABL-93 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65052 targets CD107b in IF applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glycoproteins purified from BALB/c mouse embryo 3T3 cell line
• Full Name: lysosomal-associated membrane protein 2
• GenBank Accession Number: BC138718
• Gene Symbol: CD107b
• Gene ID (NCBI): 16784
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570nm
• Form: Liquid
• Purification Method: N/A
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis (PMID 9426697).